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Jesse Wolfstadt

Jesse Wolfstadt

Hello all!

My name is Jesse Wolfstadt and I am a fourth year Kinesiology and Health Science student at York University. I have been helping out in Dr. Hawke’s lab for over a year. My previous research experience includes work at the Baycrest Centre for Geriatric Care in the Kunin-Lunenfeld Applied Research Unit. As well, I completed a research paper under Dr. Hawke’s supervision this past summer in the area of Diabetic Myopathy. I look forward to contributing to this year’s Antarctica expedition with Polar Science and helping to answer any questions you may have.

Jesse_wolfstadt's Recent Blog Entries

Answer to Week #8 Lab Team Challenge Question

Posted By Jesse_Wolfstadt, Dr. Hawke on Fri Dec 8 11:26:31 2006

Hey Polar Scientists!

I hope you have all had enough time to answer last weeks' challenge question: based on what you have learned about the feeding havits of the Weddell seal and muscle physiology in general, why do you think the Weddell seal muscle has a high percentage of Type I, slow twitch fibres? Why would these animals also have some Type IIa intermediate muscle fibres?

Weddell seals get their food by diving for long periods of time below the surface of the ice. Thus, they need to.....

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Week 8 Report from the Lab Team

Posted By Jesse_Wolfstadt, Dr. Hawke on Thu Nov 30 18:31:09 2006

Jesse in the lab

Greetings Polar Scientists!

It is a very exciting time here in the Hawke Lab at York University, as we have started our sectioning and staining of the Weddell seal muscle. In this week's update I discuss my progress over the last few weeks. I have performed serial Metachromatic staining on the mouse tissue, sectioned Weddell seal muscle, completed my first Oil-Red-O and Metachromatic stains on the seal tissue, and learned how to.....

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Answer to Lab Team Challenge Question #10

Posted By Jesse_Wolfstadt, Dr. Hawke on Fri Nov 17 09:39:52 2006

Greetings Polar Scientists!

Excellent work on the week #5 challenge question: Based on the physiology of muscle fibre types discussed in my last update, (Week #2 Report from the Lab Team), why would slow twitch muscle fibres have more intramuscular fat than fast twitch fibres? (See the Week #5 Report from the Lab Team below.)

There are three stages to cellular respiration, which is the process by which your cells make ATP: 1) Glycolysis, 2) Kreb's Cycle, and 3) Electron Transport Chain.....

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Link to Word Jumble

Posted By Jesse_Wolfstadt, Dr. Hawke on Wed Nov 15 09:21:56 2006

The link to the word jumble didn't seem to work, so here it is again: Link text

-- Jesse

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Polar Science Word Jumble

Posted By Jesse_Wolfstadt, Dr. Hawke on Wed Nov 15 09:20:29 2006

Hey Polar Scientists!

I have created a fun word jumble for everyone to try. It includes key words that I discussed in Update #5. You can see the answers right after you complete the jumble, which you can access at this link:

Enjoy!

Jesse Wolfstadt

Dr. Hawke Lab Team

York University

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Week 5 challenge Question from the Lab Team

Posted By Jesse_Wolfstadt, Dr. Hawke on Wed Nov 8 20:02:35 2006

We've posted our Week #5 Report in the Expedition News focus area.

Questions Here's this week's Challenge Question.

Based on the physiology of muscle fibre types discussed in

my last update, why would slow twitch muscle fibres have more intramuscular fat than fast twitch fibres?

Send your answers to the Challenge Questions messages area.

Good luck!

Jesse @.....

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Week 5 Report from the Lab Team

Posted By Jesse_Wolfstadt, Dr. Hawke on Wed Nov 8 19:02:10 2006

Greetings Polar Scientists!

It has been a busy week in the Hawke Lab at York University, and I am very excited to tell you about my experiences. Peter, Sophia, and I have been learning the staining and immunohistochemistry procedures that we will be using to study Weddell seal muscle. In this week's update, I will describe the two procedures that I will be using: Oil-Red-O and Metachromatic staining. As you might recall from previous updates, Oil-Red-O is used to identify intramuscular fat.....

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Learning Oil-Red-O Staining

Posted By Jesse_Wolfstadt, Dr. Hawke on Wed Nov 1 07:11:38 2006

Boo!

I hope everyone had a fun, safe, and happy Halloween yesterday.

Sophia and I had our first try at the Oil-Red-O staining procedure yesterday and today. It is a long process because we need to incubate the slides overnight once they have been stained with Oil-Red-O. I will expand on our experience in my next update.

Stay tuned!

Jesse Wolfstadt

Dr. Hawke Lab Team, York University

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Answers to Week #2 Lab Team Challenge Questions

Posted By Jesse_Wolfstadt, Dr. Hawke on Sun Oct 29 06:29:28 2006

Hey everyone!

I hope you have had enough time to give the Week 2 challenge questions a try. Here are the answers to all three questions. Thanks to everyone who participated and gave some insightful answers.

Question #1: Why does water expand as it freezes?

Answer #1: Good work students of the Alberta Rockies, although I must remind you that Wikipedia is not always the greatest source of information on the internet. Water is a very unique compound because it expands as it freezes,.....

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Upcoming Week

Posted By Jesse_Wolfstadt, Dr. Hawke on Sun Oct 29 03:53:21 2006

Greetings Polar Science Students,

All this week in the Hawke Lab at York University, I will be learning how to perform the two staining procedures for my experiments. Dr. Hawke will be teaching me how to do the Oil-Red-O and Metachromatic stains, so stay tuned for my update at the end of the week to see some new pictures of mouse muscle stained with Oil-Red-O and Metachromatic.

Have a safe and fun Halloween!

Jesse Wolfstadt

Dr. Hawke Lab, York University

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Week 2 Challenge Questions from the Lab Team

Posted By Jesse_Wolfstadt, Dr. Hawke on Tue Oct 17 11:28:52 2006

Hi everyone,

We've come up with three new Challenge Questions this week.

Read the Lab Team's Week 2 Report in the Expedition News focus area and then tackle our questions.

questions

Challenge Question #4:

Why does water expand as it freezes?

Challenge Question #5:

Why do slow twitch muscle fibres have a red colour? If you are eating chicken for dinner, and you ask for a piece of dark meat, what kind of muscle fibre are you eating: fast.....

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Week 2 report from the Lab Team

Posted By Jesse_Wolfstadt, Dr. Hawke on Tue Oct 17 10:43:42 2006

Greetings! It’s Jesse, here with the second update from the Hawke Lab Team at York University. Peter did an excellent job of describing his project in the first update, and I plan to shed some light on my project in this update.

Can you guess what this machine is?

Cryostat

Read my update to see how I've been using it to perfect my sectioning techniques.

Visit the "Expedition News" focus area and read the.....

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