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Posted By: Thomas_Hawke, Dr. Hawke on Friday, November 24, 2006
Hello Shane,
We are having problems having the Myoglobin antibody effectively staining our mouse muscle samples. However, we tried it on mouse heart (very rich in Myoglobin as you know) and we had success! The staining Sophia did looks great.
We are now going to try it on the muscle sample again using the procedure that worked on the heart sample. Wish us luck! Do you have any suggestions of why it is not working in the Western analysis?
As far as your red blood cell (RBC) contamination in the pup cultures, that is interesting! You must have some progenitor (stem) cells in your sample that is making the RBCs! Interesting, but not useful for you!
If you wash your culture for about 1-2 min in a hypotonic solution (0.87% ammonium chloride should work nicely) you will lyse the RBCs.
Hope this helps!
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